Worked example
Add 5 mL of water to a vial holding 500 mg of NAD+. The concentration is 500 ÷ 5 = 100 mg/mL, or 100,000 mcg in every millilitre. On a U-100 syringe, where 100 units make 1 mL, each unit holds 1 mg and each 10 units hold 10 mg.
Change the water and only the concentration changes. With 10 mL instead, each 10 units hold 5 mg, half as much, and any given mass takes twice the units to measure. These numbers illustrate the arithmetic; they are not amounts to use.
Concentration table
Common NAD+ vial sizes against common water volumes. The last column is what 10 units on a U-100 syringe hold.
| Vial | Water | Concentration | 10 units (U-100) hold |
|---|---|---|---|
| 100 mg | 2 mL | 50 mg/mL | 5 mg |
| 100 mg | 5 mL | 20 mg/mL | 2 mg |
| 100 mg | 10 mL | 10 mg/mL | 1 mg |
| 500 mg | 2 mL | 250 mg/mL | 25 mg |
| 500 mg | 5 mL | 100 mg/mL | 10 mg |
| 500 mg | 10 mL | 50 mg/mL | 5 mg |
| 1,000 mg | 2 mL | 500 mg/mL | 50 mg |
| 1,000 mg | 5 mL | 200 mg/mL | 20 mg |
| 1,000 mg | 10 mL | 100 mg/mL | 10 mg |
Notes for NAD+ vial math
- NAD+ masses are in the tens to hundreds of milligrams, so results often exceed a 1 mL syringe. When that happens, read the mL result rather than units; the calculator flags it.
- 500 mg in 5 mL is 100 mg/mL. One U-100 unit then holds 1 mg. At 1,000 mg in 5 mL it is 200 mg/mL, 2 mg per unit.
- NAD+ powder can take longer to dissolve than small peptides, and larger diluent volumes may not fit in a small vial. Check vial headspace before choosing a volume.
Storage and handling
- NAD+ is sensitive to light and heat and degrades in solution, faster at higher temperatures and pH. No validated in-use stability data exists for research vials.
- General practice: keep sealed vials cold and dark; refrigerate reconstituted solution, protect from light, and label with date and concentration.