The math for this vial
- Concentration: 500 mg ÷ 5 mL = 100 mg/mL (100,000 mcg/mL).
- Volume of one U-100 unit: 1 ÷ 100 = 0.01 mL.
- Mass in one unit: 100,000 mcg/mL × 0.01 mL = 1 mg.
- The whole vial is 500 U-100 units of solution, more than one 1 mL syringe holds.
On a U-40 syringe each unit is 0.025 mL, so each unit holds 2.5 times as much: 2.5 mg at 5 mL. See U-100 vs U-40 units.
Unit chart
What common U-100 readings hold from a 500 mg vial, by water volume.
| Units | mL | 2 mL water | 5 mL water | 10 mL water |
|---|---|---|---|---|
| 1 | 0.01 | 2.5 mg | 1 mg | 500 mcg |
| 5 | 0.05 | 12.5 mg | 5 mg | 2.5 mg |
| 10 | 0.1 | 25 mg | 10 mg | 5 mg |
| 20 | 0.2 | 50 mg | 20 mg | 10 mg |
| 25 | 0.25 | 62.5 mg | 25 mg | 12.5 mg |
| 50 | 0.5 | 125 mg | 50 mg | 25 mg |
| 100 | 1 | 250 mg | 100 mg | 50 mg |
About this vial size
500 mg is a mid-size NAD+ vial. Across the water volumes above, one U-100 unit holds between 500 mcg (at 10 mL) and 2.5 mg (at 2 mL). Compared with a 100 mg vial at the same water volume, it is 5 times as concentrated, so the same mass takes 0.2 times the units. A 1,000 mg vial at the same water volume is 2 times as concentrated as this one.
NAD+ masses are in the tens to hundreds of milligrams, so results often exceed a 1 mL syringe. When that happens, read the mL result rather than units; the calculator flags it.
Storage and handling
- NAD+ is sensitive to light and heat and degrades in solution, faster at higher temperatures and pH. No validated in-use stability data exists for research vials.
- General practice: keep sealed vials cold and dark; refrigerate reconstituted solution, protect from light, and label with date and concentration.
NAD+ is a coenzyme, not a peptide. There is no FDA-approved injectable NAD+ product, and research-grade vials are not for human use.